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INGREDIENTS AND FLAVOURS

Oxygen affects shelf life of veg carotenoids stored in powdered form
Thursday, 01 August, 2019, 08 : 00 AM [IST]
Vienna
The shelf life of vegetable carotenoids when stored in powdered form is severely affected by oxygen in the storage environment – an effect that has a big impact on the food industry, but that can be dramatically reduced by implementing optimised production processes.

This is the clear finding of an elaborate study using the know-how and equipment of EQ BOKU, a facility that provides precision scientific instrumentation and expertise to members of the University of Natural Resources and Life Sciences, Vienna, as well as to external clients.

The research, supported by the world’s leading manufacturer of colouring plant concentrates, compared different processes and formulations in the production of pigment-rich carrot concentrate powder, and tested their effects on storability.

The results are of major significance to the food industry, since colouring concentrates from fruits and vegetables are increasingly being used as an alternative to artificial colourings. The results of the study have now been published in Food Research International.

Vegetable concentrates sought-after
Carrot concentrates are rich in valuable carotenoids (special pigments with antioxidant effects), and highly sought-after in the food industry for use as colourings.

But in order for the rich orange colour in carrots to be transferred to food and drink preparations, the structure of the natural pigments must be preserved, and contact with oxygen avoided until they are used.

A team led by Henry Jäger, professor, Institute of Food Technology at the University of Natural Resources and Life Sciences, Vienna (BOKU), has investigated how this can now be better achieved for powder products through optimised production processes.

For the investigation, they used the cutting edge equipment available at EQ BOKU, which enables analysis of food technology process steps along entire production cycles.

Oxygen infiltration
The team made new discoveries concerning the way oxygen can infiltrate them. These insights will enable the shelf life of carotenoids in stored end products to be greatly increased.

But it is important to take advantage of the opportunities to influence the structure of the material for storage right at the beginning of the production process for vegetable concentrate powders.

“The infiltration of oxygen present in the air into pigment-rich powder particles is actually the biggest factor influencing the shelf life of colouring that we identified,” said Klara Haas, researcher from the working group.

“But with delicate management of the drying process in production, the shape of the powder particles can be determined in order to greatly reduce diffusion of oxygen into the stored goods,” she added.

A key role is played here by so-called carrier materials, which are used in production to ensure that powders have a stable structure.

In the study – which has now been published – maltodextrin, which is often employed as a carrier material, was replaced by gum arabic or modified starch.

There were good reasons for this, as the research results showed.

The carrier material can enclose a large proportion of carotene and so shield it from oxygen, but a certain amount remains on the surface and is exposed to the oxygen,” said Jäger.

“We discovered that when maltodextrin is used, after one drying stage over a third of the sensitive carotene is found on the surface of the powder particles, unprotected,” he added.

“This is reduced to just a quarter or a fifth when modified starch or gum arabic is used. This means that both are carrier materials that provide better protection against oxygen,” Jäger said.

The method of powder manufacture also has an enormous influence on storability – with the physical barriers to oxygen again proving decisive. If the powder is produced by means of freeze drying, powder particles contain micropores and nanopores that allow oxygen to penetrate quickly.

But by using an optimised spray drying process, which produces denser particles with lower surface area, oxygen diffusion during storage is reduced and the stability of the plant-based colouring concentrate is massively increased. The ideal size for particles is up to a little over 90 micrometres.

“Larger particles mean that there is a greater proportion of carotenoids inside the particles compared to that on the surface, so there is greater protection against oxygen,” said Jäger.

Antioxidants
To a certain extent, the oxidation of vegetable carotenoids can also be reduced by adding antioxidants such as tocopherol, ascorbyl palmitate and sodium ascorbate.

However, the new findings reveal the limitations of these costly measures, as Haas asserted, “Of course the antioxidants help, but if the powder particles do not have an appropriate microstructure, their effect is limited.”

“These additives can only develop their full protective capabilities when the structure physically obstructs the diffusion of oxygen through the powder,” she added.

“In a particle with the right structure, the antioxidants that are already present from the carrot are sufficient to protect the carotenoids,” Haas said.

In summary, the findings show that the shelf life of vegetable colouring concentrates when stored in powdered form can be markedly increased if an optimised drying process is used in production and modified starch or gum arabic is employed.

Without these measures, in extreme cases almost 90 per cent of carotene is lost within three months; with optimised production, this was reduced to less than 10 per cent.

Precise analysis of the storability of natural carotenoids and differing production variants was made possible by EQ BOKU’s comprehensive range of equipment.

This allows alternative production processes to be carried out at pilot scale, and results to be directly evaluated using state-of-the-art analysis equipment.
 
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